Development and characterization of monoclonal antibodies against WbSXP-1 for the detection of circulating filarial antigens.
Identifieur interne : 004F54 ( Main/Exploration ); précédent : 004F53; suivant : 004F55Development and characterization of monoclonal antibodies against WbSXP-1 for the detection of circulating filarial antigens.
Auteurs : S. Janardhan [Inde] ; P. Pandiaraja ; V. Pandey ; A. Karande ; P. KalirajSource :
- Journal of helminthology [ 1475-2697 ] ; 2011.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antihelminthe (biosynthèse), Anticorps antihelminthe (immunologie), Anticorps monoclonaux (biosynthèse), Anticorps monoclonaux (immunologie), Antigènes d'helminthe (génétique), Antigènes d'helminthe (immunologie), Antigènes d'helminthe (sang), Filariose lymphatique (diagnostic), Filariose lymphatique (immunologie), Filariose lymphatique (parasitologie), Humains, Protéines d'helminthes (génétique), Protéines d'helminthes (immunologie), Protéines recombinantes (génétique), Protéines recombinantes (immunologie), Similitude de séquences d'acides aminés, Souris, Spécificité des anticorps, Test ELISA, Wuchereria bancrofti (immunologie).
- MESH :
- biosynthèse : Anticorps antihelminthe, Anticorps monoclonaux.
- diagnostic : Filariose lymphatique.
- génétique : Antigènes d'helminthe, Protéines d'helminthes, Protéines recombinantes.
- immunologie : Anticorps antihelminthe, Anticorps monoclonaux, Antigènes d'helminthe, Filariose lymphatique, Protéines d'helminthes, Protéines recombinantes, Wuchereria bancrofti.
- parasitologie : Filariose lymphatique.
- sang : Antigènes d'helminthe.
- Animaux, Humains, Similitude de séquences d'acides aminés, Souris, Spécificité des anticorps, Test ELISA.
English descriptors
- KwdEn :
- Animals, Antibodies, Helminth (biosynthesis), Antibodies, Helminth (immunology), Antibodies, Monoclonal (biosynthesis), Antibodies, Monoclonal (immunology), Antibody Specificity, Antigens, Helminth (blood), Antigens, Helminth (genetics), Antigens, Helminth (immunology), Elephantiasis, Filarial (diagnosis), Elephantiasis, Filarial (immunology), Elephantiasis, Filarial (parasitology), Enzyme-Linked Immunosorbent Assay, Helminth Proteins (genetics), Helminth Proteins (immunology), Humans, Mice, Recombinant Proteins (genetics), Recombinant Proteins (immunology), Sequence Homology, Amino Acid, Wuchereria bancrofti (immunology).
- MESH :
- chemical , biosynthesis : Antibodies, Helminth, Antibodies, Monoclonal.
- chemical , blood : Antigens, Helminth.
- chemical , genetics : Antigens, Helminth, Helminth Proteins, Recombinant Proteins.
- chemical , immunology : Antibodies, Helminth, Antibodies, Monoclonal, Antigens, Helminth, Helminth Proteins, Recombinant Proteins.
- diagnosis : Elephantiasis, Filarial.
- immunology : Elephantiasis, Filarial, Wuchereria bancrofti.
- parasitology : Elephantiasis, Filarial.
- Animals, Antibody Specificity, Enzyme-Linked Immunosorbent Assay, Humans, Mice, Sequence Homology, Amino Acid.
Abstract
The importance of developing effective assays to diagnose, monitor and evaluate human lymphatic filariasis has been emphasized by the World Health Organization. Presently, few immunodiagnostics are available for filarial monitoring programmes. The Wuchereria bancrofti (Wb) SXP-1 parasite protein, with 84% homology to Brugia malayi (Bm) SXP-1, was found to be highly immunogenic. WbSXP-1 is one among the diagnostic candidate molecules that were used for developing a rapid-antibody-flow-through diagnostic kit for filariasis. Studies were initiated with the aim of developing monoclonal antibodies against recombinant WbSXP-1 and prospective applications for the detection of both circulating Wb and Bm antigens in serum samples from infected individuals. The monoclones 1A6C2 of subclass IgG1k, and 2A12F8 of class IgM, specifically detected Wb and Bm microfilaria isolated from patients and did not show cross-reactivity with other filarial recombinant antigens. We anticipate that this work will address the problems faced in the rapid diagnosis of human lymphatic filariasis in endemic areas in developing countries.
DOI: 10.1017/S0022149X10000118
PubMed: 20338077
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 002B22
- to stream PubMed, to step Curation: 002B22
- to stream PubMed, to step Checkpoint: 002B22
- to stream Ncbi, to step Merge: 003A14
- to stream Ncbi, to step Curation: 003A14
- to stream Ncbi, to step Checkpoint: 003A14
- to stream Main, to step Merge: 004F90
- to stream Main, to step Curation: 004F54
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Development and characterization of monoclonal antibodies against WbSXP-1 for the detection of circulating filarial antigens.</title><author><name sortKey="Janardhan, S" sort="Janardhan, S" uniqKey="Janardhan S" first="S" last="Janardhan">S. Janardhan</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Biotechnology, Anna University, Chennai 600025, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Centre for Biotechnology, Anna University, Chennai 600025</wicri:regionArea><wicri:noRegion>Chennai 600025</wicri:noRegion></affiliation></author><author><name sortKey="Pandiaraja, P" sort="Pandiaraja, P" uniqKey="Pandiaraja P" first="P" last="Pandiaraja">P. Pandiaraja</name></author><author><name sortKey="Pandey, V" sort="Pandey, V" uniqKey="Pandey V" first="V" last="Pandey">V. Pandey</name></author><author><name sortKey="Karande, A" sort="Karande, A" uniqKey="Karande A" first="A" last="Karande">A. Karande</name></author><author><name sortKey="Kaliraj, P" sort="Kaliraj, P" uniqKey="Kaliraj P" first="P" last="Kaliraj">P. Kaliraj</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:20338077</idno><idno type="pmid">20338077</idno><idno type="doi">10.1017/S0022149X10000118</idno><idno type="wicri:Area/PubMed/Corpus">002B22</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002B22</idno><idno type="wicri:Area/PubMed/Curation">002B22</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002B22</idno><idno type="wicri:Area/PubMed/Checkpoint">002B22</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002B22</idno><idno type="wicri:Area/Ncbi/Merge">003A14</idno><idno type="wicri:Area/Ncbi/Curation">003A14</idno><idno type="wicri:Area/Ncbi/Checkpoint">003A14</idno><idno type="wicri:Area/Main/Merge">004F90</idno><idno type="wicri:Area/Main/Curation">004F54</idno><idno type="wicri:Area/Main/Exploration">004F54</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Development and characterization of monoclonal antibodies against WbSXP-1 for the detection of circulating filarial antigens.</title><author><name sortKey="Janardhan, S" sort="Janardhan, S" uniqKey="Janardhan S" first="S" last="Janardhan">S. Janardhan</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Biotechnology, Anna University, Chennai 600025, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Centre for Biotechnology, Anna University, Chennai 600025</wicri:regionArea><wicri:noRegion>Chennai 600025</wicri:noRegion></affiliation></author><author><name sortKey="Pandiaraja, P" sort="Pandiaraja, P" uniqKey="Pandiaraja P" first="P" last="Pandiaraja">P. Pandiaraja</name></author><author><name sortKey="Pandey, V" sort="Pandey, V" uniqKey="Pandey V" first="V" last="Pandey">V. Pandey</name></author><author><name sortKey="Karande, A" sort="Karande, A" uniqKey="Karande A" first="A" last="Karande">A. Karande</name></author><author><name sortKey="Kaliraj, P" sort="Kaliraj, P" uniqKey="Kaliraj P" first="P" last="Kaliraj">P. Kaliraj</name></author></analytic><series><title level="j">Journal of helminthology</title><idno type="eISSN">1475-2697</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Helminth (biosynthesis)</term><term>Antibodies, Helminth (immunology)</term><term>Antibodies, Monoclonal (biosynthesis)</term><term>Antibodies, Monoclonal (immunology)</term><term>Antibody Specificity</term><term>Antigens, Helminth (blood)</term><term>Antigens, Helminth (genetics)</term><term>Antigens, Helminth (immunology)</term><term>Elephantiasis, Filarial (diagnosis)</term><term>Elephantiasis, Filarial (immunology)</term><term>Elephantiasis, Filarial (parasitology)</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Helminth Proteins (genetics)</term><term>Helminth Proteins (immunology)</term><term>Humans</term><term>Mice</term><term>Recombinant Proteins (genetics)</term><term>Recombinant Proteins (immunology)</term><term>Sequence Homology, Amino Acid</term><term>Wuchereria bancrofti (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antihelminthe (biosynthèse)</term><term>Anticorps antihelminthe (immunologie)</term><term>Anticorps monoclonaux (biosynthèse)</term><term>Anticorps monoclonaux (immunologie)</term><term>Antigènes d'helminthe (génétique)</term><term>Antigènes d'helminthe (immunologie)</term><term>Antigènes d'helminthe (sang)</term><term>Filariose lymphatique (diagnostic)</term><term>Filariose lymphatique (immunologie)</term><term>Filariose lymphatique (parasitologie)</term><term>Humains</term><term>Protéines d'helminthes (génétique)</term><term>Protéines d'helminthes (immunologie)</term><term>Protéines recombinantes (génétique)</term><term>Protéines recombinantes (immunologie)</term><term>Similitude de séquences d'acides aminés</term><term>Souris</term><term>Spécificité des anticorps</term><term>Test ELISA</term><term>Wuchereria bancrofti (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Antibodies, Helminth</term><term>Antibodies, Monoclonal</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antigens, Helminth</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Antigens, Helminth</term><term>Helminth Proteins</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Helminth</term><term>Antibodies, Monoclonal</term><term>Antigens, Helminth</term><term>Helminth Proteins</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Anticorps antihelminthe</term><term>Anticorps monoclonaux</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Antigènes d'helminthe</term><term>Protéines d'helminthes</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antihelminthe</term><term>Anticorps monoclonaux</term><term>Antigènes d'helminthe</term><term>Filariose lymphatique</term><term>Protéines d'helminthes</term><term>Protéines recombinantes</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Elephantiasis, Filarial</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Antigènes d'helminthe</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Antibody Specificity</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Humans</term><term>Mice</term><term>Sequence Homology, Amino Acid</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Humains</term><term>Similitude de séquences d'acides aminés</term><term>Souris</term><term>Spécificité des anticorps</term><term>Test ELISA</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The importance of developing effective assays to diagnose, monitor and evaluate human lymphatic filariasis has been emphasized by the World Health Organization. Presently, few immunodiagnostics are available for filarial monitoring programmes. The Wuchereria bancrofti (Wb) SXP-1 parasite protein, with 84% homology to Brugia malayi (Bm) SXP-1, was found to be highly immunogenic. WbSXP-1 is one among the diagnostic candidate molecules that were used for developing a rapid-antibody-flow-through diagnostic kit for filariasis. Studies were initiated with the aim of developing monoclonal antibodies against recombinant WbSXP-1 and prospective applications for the detection of both circulating Wb and Bm antigens in serum samples from infected individuals. The monoclones 1A6C2 of subclass IgG1k, and 2A12F8 of class IgM, specifically detected Wb and Bm microfilaria isolated from patients and did not show cross-reactivity with other filarial recombinant antigens. We anticipate that this work will address the problems faced in the rapid diagnosis of human lymphatic filariasis in endemic areas in developing countries.</div></front></TEI><affiliations><list><country><li>Inde</li></country></list><tree><noCountry><name sortKey="Kaliraj, P" sort="Kaliraj, P" uniqKey="Kaliraj P" first="P" last="Kaliraj">P. Kaliraj</name><name sortKey="Karande, A" sort="Karande, A" uniqKey="Karande A" first="A" last="Karande">A. Karande</name><name sortKey="Pandey, V" sort="Pandey, V" uniqKey="Pandey V" first="V" last="Pandey">V. Pandey</name><name sortKey="Pandiaraja, P" sort="Pandiaraja, P" uniqKey="Pandiaraja P" first="P" last="Pandiaraja">P. Pandiaraja</name></noCountry><country name="Inde"><noRegion><name sortKey="Janardhan, S" sort="Janardhan, S" uniqKey="Janardhan S" first="S" last="Janardhan">S. Janardhan</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004F54 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 004F54 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= LymphedemaV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:20338077
|texte= Development and characterization of monoclonal antibodies against WbSXP-1 for the detection of circulating filarial antigens.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:20338077" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a LymphedemaV1
| This area was generated with Dilib version V0.6.31. |  |